| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.23[luc2-minP] |
| 质粒类型: | 哺乳细胞，信号通路报告载体 |
| 终止子: | SV40 poly(A) signal |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 复制子: | pUC |
| 载体大小: | 4283 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 备注: | 带有最小启动子的载体，用于使用荧光素酶分析测量转录反应元件的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.23[luc2-minP]载体图谱](/uploads/载体图谱/pGL4.23[luc2-minP]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.23[luc2 minP] 4283 bp ds-DNA circular SYN 29-JUN-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using a luciferase assay.
ACCESSION DQ904455
VERSION .
KEYWORDS pGL4.23[luc2 minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4283)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Monday, February 24, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4283
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..1793
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1837..1958
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2377..2965)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3165..4025)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4130..4178
/note="synthetic polyadenylation signal"
misc_feature 4192..4283
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct
1801 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
1861 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
1921 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
1981 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
2041 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc
2101 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
2161 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
2221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
2281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
2341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
2401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
2461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
2521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
2581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
2641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
2701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
2761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
2821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
2881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
2941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
3061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
3121 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
3181 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
3241 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
3301 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
3361 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
3421 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
3481 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
3541 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
3601 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
3661 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
3721 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
3781 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
3841 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
3901 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
3961 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
4021 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
4081 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
4141 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
4201 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
4261 tgcaggtgcc agaacatttc tct
//
